Part:BBa_K731600

Cysteine Desulfhydrase (CysDes)

This part encode for a cysteine desulfhydrase from Treponema denticola, which is known to be a strong enzyme that produces H2S, ammonia and pyruvate starting from cysteine. It is often indicated also as Hly.

This part was cloned by the iGEM Trento 2012 team for the creation of an aerobically engineered pathway for the removal of the black crust disfiguring marble stones. Further information about this part and its characterization can be found in the [http://2012.igem.org/Team:UNITN-Trento iGEM Trento 2012 wiki page].

SAFETY NOTES Please note that this part produces hydrogen sulfide, which is toxic if inhaled in high concentrations. Cells handling should be done under a chemical hood. A safety handbook to work with sulfate reducing bacteria is posted on the Trento 2012 [http://2012.igem.org/Team:UNITN-Trento wiki].

Usage and Biology

CysDes is a unique 45 KDa hemolysin cysteine dependent, that was shown to have also aminotransferase activity. (1, 2) The enzyme catalyzes the degradation of L-cysteine to produce hydrogen sulfide, ammonia and pyruvate.

This part has been used to create two different devices (BBa_K731400 and BBa_K731480) which were used to characterize the enzymatic activity of the gene CysDes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 399
  
• 1000
  COMPATIBLE WITH RFC[1000]